Influence of Long-Term Agar-Slant Preservation at 4 °C on the Recombinant Enzyme Activity of Engineered Yeast

Strain preservation to maintain stable vitality and the recombinant enzyme activity plays a crucial role in industrial fermentation. A Pichia pastoris strain is routinely stored at −80 °C glycerol vial activated on an antibiotic-containing YPD agar plate before being used for Alternatively, should be preserved slant 2~4 (low-temperature storage) short period use. To maximize utilization of low-temperature storage fermentation, we evaluated this method by observing capacity both different durations. We found that engineered yeast could least 30 months without losing its biomass end fermentation directly seed cultivation which more time-saving than recovery from vial. Moreover, antibiotic added omitted if heterologous gene was integrated into host chromosome. Our approach may greatly elevate production efficiency strain.